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TitelSerodiagnosis of tuberculous lymphadenitis using a combination of antigens  
AuteursD. Beyene, K.L. Franken, L. Yamuah, A. Aseffa, H.G. Wiker, A. Kolk, H. Engers, P. Klatser, L. Sviland
Jaar2010
TijdschriftJournal of Infection in Developing Countries
Jaargang4
Serienummer2
Pagina's96-102
ISSN[1972-2680]
OrganisatieKIT - Royal Tropical Institute
OnderwerpHealth and Nutrition
Trefwoordenhealth, disease prevention and control
SamenvattingBACKGROUND: The diagnosis of extra-pulmonary tuberculosis (EPTB) by conventional methods such as culture and microscopy has low sensitivity and requires an invasive procedure. A simple rapid serological test would be of great value. METHODOLOGY: Six antigens (ESAT-6, Ag85A, TB10.4, Rv3881c, lipoarabinomannan (LAM) and Ara6-BSA) were tested in an ELISA to detect antigen-specific IgG and IgM antibodies in sera from 54 culture- and histology-confirmed tuberculous lymphadenitis (TBLN) patients as follows: four were HIV seropositive; sera from 25 was smear positive for pulmonary tuberculosis (PTB); 15 were culture- and histology-negative lymphadenitis (non-TBLN) patients; and 22 werehealthy controls (HCs). RESULTS: The sensitivities of the antigens for the detection of IgG in sera of TBLN patients ranged from 4% to 30%. Specificities ranged from 73% to 100% with sera from non-TBLN patients and 91% to 100% with sera from HCs. Sensitivities of the antigens for detection of IgM ranged from 0% to 15% and specificities ranged from 80% to 100% with sera from non-TBLN patients and 91% to 100% with sera from HCs. LAM was the most potent antigen for detection of IgG. When LAM and ESAT-6 were combined, sensitivity increased up to 43% and specificity with non-TBLN was 80% with HC 96%. CONCLUSIONS: The study suggests that the combined use of LAM and ESAT-6 for IgG antibody detection in sera from TBLN patients could be a supplement to microscopy of fine-needle aspirate (FNA) to diagnose TBLN among patients suspected of TBLN.  
TaalEngels
CategorieResearch
Soort documentArtikel
Rechten© 2010 Beyene et al. This article is licensed under a Creative Commons Attribution License.
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